Calibration

Calibration

Perform the common setup in Quick Start Steps, then click Calibration to start. Continue with the steps below.

1. Hardware
   1. Set Base Temp to 20 °C and click Set Low Temp.
      • Note: The temperature set on the 4D Nano Align should change. If it does not, make sure the Chiller is on.
   2. Set High Temp to 120 °C.
      • Note: Microscope Settings are auto-filled from Project Manager (no need to reselect them).
2. Timing/Illumination
   1. Set Stage Heating Time to 50.
   2. Set Image Averages to 55.
   3. Auto-select the Brightness (%).
      • Note: Brightness % is auto-filled from Project Manager (no need to reselect it).
3. 3D Alignment – Focus ROI
   1. Select a region on the CCD image using the rectangle tool. Choose a unique feature on the sample, such as edges or scratch marks.
   2. Press Focus ROI to set the selected region as your ROI.
   3. Wait a few seconds until the selected region is focused as your ROI and the Focus ROI indicator turns green.
      • Note: The selected ROI region displays in green. To zoom in/out, use the magnifying glass — hold Shift to zoom out, Shift+Click to zoom in.
4. 3D Alignment – Capture Ref
   1. After selecting the ROI, keep the same ROI or select another region with more distinct features for alignment.
   2. Press Capture Ref to set the newly selected region as the Reference region.
   3. Wait for the Capture Ref indicator to turn green.
   4. Set the initial parameters for Focus Tolerance, Alignment %, and Vibration Filter. The indicator lights turn green when the image is in range.
      • Note: Increasing the Alignment % value and decreasing the Vibration Filter improves alignment accuracy.
5. Start Measurement
   1. Once setup is complete, click START Measurement to begin calibration.
6. Save Images
   1. The calibration cycles through temperatures to reduce noise and improve accuracy. Typically after 3 cycles, the thermoreflectance error is <1E-5 (or <5% for 530 nm on gold material). The System Status bar shows progress and displays "Calibration Done" after each cycle, and counts cycles under "Loop Iterations".
      • Note: Each cycle takes about 4 minutes.
   2. Select a region on the Thermal Image View to get the Thermoreflectance Coefficient (Cth) while the measurement is running. (Example in source: the middle of the blue 5 µm x 500 µm band is selected.)
   3. Under Save Images, check that the directory and "Save Path" name are correct.
   4. Check the Save Every Cycle box. When ready to save the data, click Save Image.
      • Note: Save the image before stopping the measurement.
   5. To stop the measurement, click STOP MEASUREMENT, or click File > Quit.